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MyBiosource Biotechnology elisa kits mouse collagen type iii
Elisa Kits Mouse Collagen Type Iii, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SMOC1 silencing mitigates the oxidative stress in MFBs induced by Ang II. <t>ELISA</t> was performed to evaluate the (A) MDA content, (B) LDH content and (C) SOD activity in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05 and **P<0.01 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; MFBs, myocardial fibroblasts; Ang II, angiotensin II; MDA, malondialdehyde; LDH, lactate dehydrogenase; SOD, superoxide dismutase; si, small interfering RNA; NC, negative control.
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SMOC1 silencing mitigates the oxidative stress in MFBs induced by Ang II. <t>ELISA</t> was performed to evaluate the (A) MDA content, (B) LDH content and (C) SOD activity in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05 and **P<0.01 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; MFBs, myocardial fibroblasts; Ang II, angiotensin II; MDA, malondialdehyde; LDH, lactate dehydrogenase; SOD, superoxide dismutase; si, small interfering RNA; NC, negative control.
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SMOC1 silencing mitigates the oxidative stress in MFBs induced by Ang II. ELISA was performed to evaluate the (A) MDA content, (B) LDH content and (C) SOD activity in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05 and **P<0.01 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; MFBs, myocardial fibroblasts; Ang II, angiotensin II; MDA, malondialdehyde; LDH, lactate dehydrogenase; SOD, superoxide dismutase; si, small interfering RNA; NC, negative control.

Journal: Oncology Letters

Article Title: SMOC1 silencing suppresses the angiotensin II-induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway

doi: 10.3892/ol.2018.8989

Figure Lengend Snippet: SMOC1 silencing mitigates the oxidative stress in MFBs induced by Ang II. ELISA was performed to evaluate the (A) MDA content, (B) LDH content and (C) SOD activity in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05 and **P<0.01 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; MFBs, myocardial fibroblasts; Ang II, angiotensin II; MDA, malondialdehyde; LDH, lactate dehydrogenase; SOD, superoxide dismutase; si, small interfering RNA; NC, negative control.

Article Snippet: The expression of TGF-β1, COL-I and COL-III were determined by ELISA kits: Mouse TGF-β1 ELISA Kit (E-EL-M0051c; Elabscience, Wuhan, Hubei, China), COL1 ELISA kit (E-EL-M0325c; Elabscience) and COL-III ELISA kit (E-EL-M0316; Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, Transfection, Plasmid Preparation, Binding Assay, Small Interfering RNA, Negative Control

SMOC1 silencing downregulates the expression levels of fibrosis-associated proteins. (A) ELISA was performed to measure the TGF-β1, COL-I and COL-III expression in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. (B) Reverse transcription-quantitative polymerase chain reaction and (C) western blot analysis were performed on the expression levels of FN, TGF-β1, COL-I and COL-III in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05, **P<0.01, and ***P<0.001 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; FN, fibronectin; TGF-β1, transforming growth factor β1; COL, collagen; Ang II, angiotensin II; MFBs, myocardial fibroblasts; si, small interfering RNA; NC, negative control.

Journal: Oncology Letters

Article Title: SMOC1 silencing suppresses the angiotensin II-induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway

doi: 10.3892/ol.2018.8989

Figure Lengend Snippet: SMOC1 silencing downregulates the expression levels of fibrosis-associated proteins. (A) ELISA was performed to measure the TGF-β1, COL-I and COL-III expression in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. (B) Reverse transcription-quantitative polymerase chain reaction and (C) western blot analysis were performed on the expression levels of FN, TGF-β1, COL-I and COL-III in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05, **P<0.01, and ***P<0.001 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; FN, fibronectin; TGF-β1, transforming growth factor β1; COL, collagen; Ang II, angiotensin II; MFBs, myocardial fibroblasts; si, small interfering RNA; NC, negative control.

Article Snippet: The expression of TGF-β1, COL-I and COL-III were determined by ELISA kits: Mouse TGF-β1 ELISA Kit (E-EL-M0051c; Elabscience, Wuhan, Hubei, China), COL1 ELISA kit (E-EL-M0325c; Elabscience) and COL-III ELISA kit (E-EL-M0316; Elabscience).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, Small Interfering RNA, Negative Control